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Normalized ANS fluorescence intensity change as a function of temperature in the presence of untreated (control) IgG1 (black line), partially deglycosylated IgG1 (green line), and fully deglycosylated IgG1 (red line) from pH 4 to 6. Normalized results were generated by fitting the data to be equal to 1 at the maxima and to 0 at the minima for incorporation into the EPDs and radar charts. Curves shown here are averages of three runs.
