Skip to main content
. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Cell Metab. 2013 Oct 1;18(4):10.1016/j.cmet.2013.09.004. doi: 10.1016/j.cmet.2013.09.004

Figure 5. Serum from resveratrol-treated monkeys on HFS diet exerts anti-inflammatory effects in 3T3-L1 adipocytes.

Figure 5

Fully-differentiated 3T3-L1 adipocytes were incubated for 24-h with media containing serum from SD and HFS +/− Resv diet-fed monkeys for 2 years. (A) SIRT1 protein levels. (B) IκBα protein levels. (C) Phosphorylated NF-κB/NF-κB ratio. Lanes were run on the same gel but were noncontiguous. (D) mRNA expression for IL-6, TNF-α, IL-1β and adiponectin. (A to D) The graphs show the mean ± SEM from 3 independent experiments, and the results are expressed as percent increase over the values observed in adipocytes treated with HFS serum. The data were analyzed using One-Way ANOVA. *, P < 0.05 (HFS vs. HFS + Resv serum); #, P < 0.05 (HFS vs. SD serum). SD: standard diet; HFS: high-fat, high-sugar; Resv: resveratrol; pNF-κB: phosphorylated NF-κB.