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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2013 Sep 9;26(6):10.1111/pcmr.12158. doi: 10.1111/pcmr.12158

Figure 1. Attenuation of UPR signaling in Oca2-null melanocytes under routine culture conditions.

Figure 1

Protein and RNA were extracted from melanocytes and subjected to immunoblot analysis and reverse transcriptase PCR, respectively, to determine (a) Ire1 protein expression and Xbp-1 mRNA splicing, and (b) expression of key proteins involved in Perk signaling. Xbp1-u= unspliced Xbp-1; Xbp1-s=spliced Xpb-1. A faint higher molecular band reported as representing a hybrid amplicon is also observed (Back et al., 2005).