Figure 1.

Reprogramming of transcription factor expression caused by flagellin pretreatment in primary HCECs. Normal or flagellin-pretreated (50 ng/mL, 24 hours) cells were challenged with live PAO1 (1:50 MOI) for 4 hours, and transcription factor RNA expression alterations were quantified by comparing RNA fold changes of PAO1-infected normal cells versus PAO1-infected flagellin-pretreated cells using the real-time RT² Profiler PCR Array (A). For confirmation of PCR array results, cells were cultured and challenged under the same conditions and lysed and subjected to Western blotting analyses with IRF1 and β-actin antibodies (D). Cell culture media were collected 4 hours after bacterial challenge for the analyses of IL-8 by ELISA (B) and hBD2 using slot blot (C). P values were generated using unpaired Student's t-test (**P < 0.01). The figure is a representative of four independent experiments.