Figure 3.

Distribution of CXCL10 expression in corneal epithelia of WT and Irf1^−/− mice. WT and Irf1^−/− B6 mice were topically pretreated with 500 ng flagellin in 5 μL PBS, a dosage we showed to induce protection in B6 mice, or 5 μL PBS for 24 hours, and then infected with PAO1 at 10⁵ CFU for 6 hours. Mouse eyes were enucleated in OCT, sectioned, stained with mouse anti-CXCL10 antibody, and examined with fluorescence microscopy middle panels with DAPI used to stain the nuclei (A, B, D, G, J, K, M, N, P, Q) and ×4 lens to view the entire mouse corneas (insert [i] in [E, H) or confocal microscopy (C, E, F, H, I, L, O, R). Scale bars are given in the figures.