Figure 7.

Mc4r expression analysis. (A) RT-PCR analysis in Xiphophorus using a reverse primer matching the “classical” 3′ UTR (“mc4r”) or the alternative 3′ UTR [“mc4r (3′ UTR*”)]. Expression of the single copy mc4r of the medaka O. latipes was also analyzed. For calibration, the cDNA amount used in the PCR reaction was monitored with actin PCR. “no RT” indicates treatment without reverse transcriptase, indicating absence of contaminating genomic DNA. (B) Northern blot analysis in Xiphophorus using an internal mc4r fragment from X. maculatus as a probe.