Effects of treatment, in conjunction with estrogen antagonist ICI 182 780, with E2 (top), genistein (middle), and naringenin (bottom) on luciferase gene expression in T47D-KBluc cells cultured in cs-FBS-supplemented medium. Values are expressed as (mean value of induction as a multiple of the value of the vehicle control of four replicate assays) ± (standard error of the mean). The level of luciferase activity was significantly different from the activity revealed following treatment with 1 μM 17β-estradiol, 5 μM genistein or 5 μM naringenin (P<0.05). E2, 1 μM 17β-estradiol; E2+I (0.1, 1, and 10), 1 μM 17β-estradiol with ICI 182 780 (0.1, 1, and 10 μM); G only, 5 μM genistein; G+I (0.1, 1, and 10), 5 μM genistein with ICI 182 780 (0.1, 1, and 10 μM); N only, 5 μM naringenin; N+I (0.1, 1, and 10), 5 μM naringenin with ICI 182 780 (0.1, 1, and 10 μM).