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. 2013 Nov 18;8(11):e80397. doi: 10.1371/journal.pone.0080397

Figure 3. Treatment-resistant GSC clones are slow-cycling cells express upregulated stem cell markers.

Figure 3

A. Total RNA of sensitive clones and resistant clones were extracted. The mRNA expression levels of indicated stem cell-associated genes were analyzed by semi-qtRT-PCR with specific primers. β-actin was used as an internal control gene. B. Proliferative activity of cells populated by resistant clones and sensitive clones was determined by 3-day MTS/PMS cell proliferation assays. Data represent mean values ± SD of triplicate measurements. C. Sensitive clones and resistant clones were pulsed with 5-bromo-2-deoxyuridine (BrdU) for 1 hr, and cell cycle of BrdU+ cells was analyzed 3 hrs and 6 hrs after BrdU pulsing. Cell cycle phases were defined by 7-amino-actinomycin (7-AAD) staining intensities.