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. 2013 Nov 18;8(11):e80397. doi: 10.1371/journal.pone.0080397

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© 2013 Ye et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Cells derived from treatment-resistant GSC clones were treated with RT (12 Gy or 1.5 Gy) in the presence or absence of indicated siRNA targeting selected signatures of resistant clones. Photos were taken 7 days after treatment. B. Verification of on-target gene knockdown by indicated siRNA treatment. Total RNA from the resistant clones treated with specific siRNA was extracted. The mRNA expression levels of indicated genes were analyzed by qtRT-PCR with specific primers. β-actin was used as an internal control gene.