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. 2013 Nov 18;8(11):e80470. doi: 10.1371/journal.pone.0080470

Figure 1. TRAF3 interacts with Ubc9 in a yeast 2-hybrid assay.

Figure 1

(A) S. cerevisiae strain PJ69-4A was transformed with plasmids expressing the GAL4 activation domain (AD) fused to Ubc9, the GAL4 binding domain (BD) fused to TRAF3 or with control empty vectors in all possible combinations. Cells were grown on standard medium (SD-leu-trp) in the presence or absence of X gal (SD-leu-trp+X gal), medium lacking adenine (SD-leu-trp-ade) to detect expression of the GAL-ADE2 reporter gene or medium lacking histidine (SD-leu-trp-his + 5 mM 3-AT) to detect expression of the GAL-HIS3 reporter gene. (B) Quantification of galactosidase reporter activity in yeast transformed with the vectors described in (A). Results shown represent the mean values of galactosidase activity (±SD) relative to controls from 4 independent experiments.