Table 4. PCR amplification.

	Name	Year	Serotype	lacC	lacE (J1)	lacG (J1)	lacE (J2)	lacG (J2)	sorC	sorE	GCK	ptsG	cas1
Korean strains	KCTC11537 (ref)	2006	I	-	-	-	-	-	+	+	+	+	-
	KCTC11538	2008	I	-	-	-	-	-	+	+	+	+	-
	J19	2009	I	-	-	-	-	-	+	+	+	+	-
	J20	2009	I	-	-	-	-	-	+	+	+	+	-
	J24	2009	I	-	-	-	-	-	+	+	+	+	-
	J25	2009	I	-	-	-	-	-	+	+	+	+	-
	J21	2009	II	-	-	-	-	-	+	+	+	+	+
	J22	2009	II	-	-	-	-	-	-	-	+	+	+
	J23	2009	II	-	-	-	-	-	-	-	+	+	+
	J27	2009	II	+	-	-	+	+	-	-	+	+	+
	J28	2009	II	-	-	-	-	-	-	-	+	+	+
	J30	2009	II	-	-	-	-	-	-	-	+	+	+
Japanese strains	KRS-02083	2002	I	+	+	+	-	-	-	-	+	+	-
	KRS-03032	2003	I	-	-	-	-	-	+	+	+	+	-
	KRS-02030	2002	I	+	+	+	-	-	-	-	+	+	-
	KRS-04024	2004	I	-	-	-	-	-	+	+	+	+	-
	KRS-02067	2002	I	+	+	+	-	-	+	+	+	+	-
	KRS-04037	2004	I	-	-	-	-	-	+	+	+	+	-
	KRS-02109	2002	II	+	-	-	+	+	-	-	+	+	+
	KRS-02068	2002	II	+	-	-	+	+	-	-	+	+	+
	KRS-02090	2002	II	+	-	-	+	+	-	-	+	+	+
	KRS-02087	2002	II	+	-	-	+	+	-	-	+	+	+
	KRS-02091	2002	II	+	-	-	+	+	-	-	+	+	+
	KRS-02102	2002	II	+	-	-	+	+	-	-	+	+	+

Lactose-, sorbose- and glucose-related genes, along with gene markers of the CRISPR/Cas system, were PCR amplified from 24 stored S. parauberis strains that had been isolated from diseased olive flounder in Korean (six strains each of type I and type II) between 2006 and 2009, and Japan (six strains each of type I and type II) between 2002 and 2004.

Symbols: ‘-’ indicates no amplification; ‘+ ’ indicates amplification.