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Experimental setup. MAC-T cells (1) and macrophages (2) were cultured separately or co-cultured (7 and 8, respectively) at a density of ~ 2.0 × 104 cells/mL under uninfected (1, 2, 7 and 8) or MAP infected conditions (4, 6, 10 and 11). RNA was extracted from host cells (1, 2, 4, 6, 10 and 12) and pathogen located inside MAC-T cells (3 and 9) and macrophages (5 and 11) at 30 min post-infection. A total of 12 conditions (samples) were submitted for RNA-seq analysis.
