Figure 1. Determination of the amount of exogenous β-glucosidase required to exert an antifungal effect from esculin (3402.8 µg/ml).

The negative control containing 348.36 µg/ml esculetin + 0.005 U/ml β-glucosidase demonstrated total kill for both strains. The positive control contains 0.005 U/ml β-glucosidase but no esculin and resulted in no growth inhibition. β-glucosidase at all concentrations tested demonstrated no antifungal activity. All results are representative of triplicate samples from triplicate experiments. Error bars represent the standard deviation of the mean.