Figure 3. SDS-PAGE and Western blot analysis.

Eluted fractions (1-3) from the representative purification of FcRn_ECD were analyzed by SDS-PAGE. The gel was stained with GelCode Blue Stain Reagent or was silver stained and showed essentially only two bands with a molecular mass of 36 and 12 kDa. WB: western blotting of pooled fractions using antibodies specific for FcRn and human β₂m, identifying the protein bands as αFcRn_ECD and β₂m.