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4EASO transfection of H2373 cells resulted in reduced expression of eIF4E and eIF4E-regulated proteins Bcl-2 and ODC as demonstrated by Western analysis. eIF4E and Bcl-2 levels were diminished in H2596 cells by 4EASO treatment. Lysates were prepared 72 hours following treatment with mmASO and 4EASO. β-actin serves as a loading control.
