Figure 1. Developmental pathways of pre-treated wheat microspores in culture, as determined by time-lapse tracking.

According to the type of development, microspores are grouped into three classes: type I (A–H), type II (I–O) and type III (P–R). Microspores were stained with FM 4–64 (Molecular Probes Cat. # T-3166) to confirm their viability in culture. All pictures were taken at a 25× magnification and 6× optical zoom except for G, O and H, where the former two pictures were taken at the same magnification but at 3× optical zoom and the latter picture was taken at 10× magnification and 1.7× optical zoom.