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After incubation with and without 250pg/ml recombinant CXCL1 for 16 hours, human HSC were stained for α-SMA and Collagen type I and analysed by flow cytometry. This representative set of histograms shows that α-SMA and Collagen type I expression increases after CXCL1 stimulation (solid lines) as compared to the unstimlated controls (dotted line).
