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. 2013 Nov 18;8(11):e79327. doi: 10.1371/journal.pone.0079327

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© 2013 Zahedi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a. The magnitude of diuresis in rats co-treated with ACTZ and HCTZ but without ACTZ pretreatment was significantly less than in rats pretreated with ACTZ followed by ACTZ and HCTZ co-treatment. b, c and d. Immunofluorescent microscopic analysis of pendrin in control (b), simultaneous treatment with ACTZ and HCTZ (c) and sequential treatment with ACTZ and HCTZ (d).