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. 2013 Nov 18;8(11):e78617. doi: 10.1371/journal.pone.0078617

Figure 6. CFHR2 exerts neither decay acceleration nor cofactor activity.

Figure 6

(A) CFHR2 can not dissociate factor B from the in vitro assembled convertase C3bBb, but factor H efficiently decays factor Bb fragment from C3b. (B) CFHR2 does not influence cleavage of C3b bound factor B by factor D. CFHR2 preincubated NHS, which was activated by surface immobilized IgM via the AP and Ba cleavage was followed by ELISA. A representative experiment is shown. (C) CFHR2 alone lacks cofactor activity for factor I to degrade C3b (lanes 6–8). CFHR2 does not interfere with factor H cofactor activity (lanes 2–5). Increasing amounts of CFHR2 (1–100 µg/ml) were incubated with C3b and factor I in the presence or absence of factor H. Reaction mixtures were separated by SDS PAGE and immunoblotted using a C3 polyclonal antibody.