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. 2013 Nov 18;8(11):e79333. doi: 10.1371/journal.pone.0079333

Figure 2. Elution profile of crude extract CM-H-L from Sephadex LH-20.

Figure 2

Fraction collected through elution with 100% ethanol, 100 ml of 60% ethanol, 100 ml of 100% ethanol, corresponding to the strand on the x axis between 0 and 100 ml, 100 and 200 ml, 200 and 300 ml, 300 and 400 ml respectively. Sephadex LH-20 yielded five fractions at OD 260 nm, CM-H-L-1, CM-H-L-2, CM-H-L-3, CM-H-L-4 and CM-H-L-5.