Table 1. List of primers for real-time PCR.
Namea | Sequences (50-30)b |
ORF7-F1 | AATAACAACGGCAAGCAGCA |
ORF7-R1 | GCACAGTATGATGCGTCGGC |
IFN-α-F1 | AGAGCCTCCTGCACCAGTTCT |
IFN-α-R1 | CTGCATGACACAGGCTTCCA |
IL-1β-F1 | TCTGCCCTGTACCCCAACTG |
IL-1β-R1 | CCCAGGAAGACGGGCTTT |
IFN-β-F1 | AGCACTGGCTGGAATGAAACCG |
IFN-β-R1 | CTCCAGGTCATCCATCTGCCCA |
GAPDH-F1 | CCTTCCGTGTCCCTACTGCCAAC |
GAPDH-R1 | GACGCCTGCTTCACCACCTTCT |
TNF-α-F1 TNF-α-R1 | CCCCCAGAAGGAAGAGTTTC CGGGCTTATCTGAGGTTTGA |
F1: forward primer, R1: reverse primer.
Swine gene sequences were downloaded from GenBank.