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. 2013 Nov 18;8(11):e79671. doi: 10.1371/journal.pone.0079671

Figure 2. TET inhibits C. albicans SC5314 biofilm formation in vitro.

Figure 2

(A) Effects of different concentrations of TET on biofilm formation. (B) Effects of different concentrations of Amphotericin B on biofilm formation. AmB: amphotericin B. (C) Effects of different concentrations of TET on the maintenance of mature biofilms. Biofilm formation was evaluated by XTT reduction assay, and the results were presented as the percentage compared to the control biofilms formed without TET treatment. Biofilm formation results represent the mean ± standard deviation for five independent experiments. * P<0.05 compared to the control biofilms, ** P<0.01 compared to the control biofilms. (D) Effects of different concentrations of TET on biofilms formed on silicone pads. Standard deviations are depicted and based on 6 silicone pad measurements. ** P<0.01. (E) Screen for TET-treated biofilms formed on silicone pads. The wells are shown for a: normal biofilm. b: cells were treated with 4 mg/L TET. c: 8 mg/L TET. d: 16 mg/L TET. e: 32 mg/L TET. f: uninoculated control. (F) Effects of different concentrations of TET on biofilm formation presented by using CLSM. a: Control. b: 8 mg/L of TET. c: 16 mg/L TET. d: 32 mg/L TET. (G) Effects of different concentrations of TET on biofilm formation presented by using SEM. The inset in the 500 ×, 2000× panels show the area that was magnificated.