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. 2013 Nov 18;8(11):e79845. doi: 10.1371/journal.pone.0079845

Figure 2. Tbx3 mRNA expression in Gli3xt/xt embryos.

Figure 2

Whole-mount in situ hybridization for Tbx3 in wt (A, B) and Gli3xt/xt (C, D) embryos. At the 40-somite stage, wt embryos (A) express Tbx3 mRNA in the mesenchyme between the fore- and hind-limb. This band of expression is narrower in Gli3xt/xt embryos at this stage (yellow bracket). At the 45-somite stage Tbx3 is reduced within the mesenchyme but is induced within the epithelium of developing mammary placodes #3 (white arrow) in wt embryos (B). Gli3xt/xt embryos (D), show a narrower band of Tbx3 expression between the fore- and hind-limb and fail to concentrate epithelial expression within placode #3 (white arrow). The control in situ hybridization for Tbx3 sense probe is shown in (E). Abbreviations: F – fore-limb, H – hind-limb, s – somite.