Figure 4. Evagination of MR#2 and encroachment of hair follicles in Gli3 ^xt/xt embryos.

Analysis of outer surface of E18.5 skin whole-mounts (A–D) stained with carmine (C, D) and sections stained with nuclear fast red (E, F) and X-Gal (blue) to detect hair follicles expressing the Gli1-LacZ reporter (A–F). MR#1 (A) from Gli3^xt/xt; Gli1^lzki/+ mutants and MR#2 from control Gli1^lzki/+ (C) embryos show normal invagination and appropriate exclusion of hair follicles. In contrast MR#2 from Gli3^xt/xt; Gli1^lzki/+ mutants (B, D) showed prominent evagination and encroachment of hair follicles.