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MDA-231 and MCF10A cells were cultured in normal glucose (5 mM) or high glucose (10 mM) for 24 hr and then subjected to immunofluorescence staining for leptin receptor (left panel, green) and active JAK2 (middle panel, red). Images merged with DAPI reveal the level of colocalization between leptin receptor and JAK2 within individual cells (right panel).
