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Bias in the estimate of the number of segregating sites (left panel) and the expected heterozygosity (right panel) under different experimental scenarios. Sequencing depths are 
, 
, 
, and 
and the corresponding sample sizes are 
, 
, 
, and 
individuals. I simulated 100 regions of 
independent sites, with a probability of each site being variable in the population equal to 0.1.
