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. 2013 Nov 18;8(11):e79433. doi: 10.1371/journal.pone.0079433

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© 2013 Foster et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Examples of vacuolation and immunostaining for PrP^Sc in brain tissue. A. Haematoxylin and Eosin (H&E) staining showing vacuolation in the dorsal motor vagus nucleus of L55 (VRQ/VRQ) had natural scrapie-like disease (x100). B. Strong immunostaining for PrP^Sc (diagnostic for natural scrapie) with BG4 antibody in the dorsal motor vagus nucleus of L55 (x40). C. Marginal immunostaining for PrP^Sc (diagnostic for SSBP/1) with BG4 in the dorsal motor vagus in the brain of L111 (VRQ/ARR) (x 100). D. Marginal immunostaining for PrP^Sc (diagnostic for SSBP/1) with BG4 in the dorsal motor vagus of L101 (VRQ/VRQ) (x100). E. Nil vacuolation (typical for SSBP/1) in an H&E section of the dorsal motor vagus in the brain of L101 (VRQ/VRQ) (x100). F. Marginal immunostaining for PrP^Sc (diagnostic for SSBP/1) in the brain of L106 (VRQ/VRQ) (x100).