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. 2013 Nov 8;8(11):e79418. doi: 10.1371/journal.pone.0079418

Figure 5. Sch B in the effects of myocardial cell apoptosis.

Figure 5

(A) TUNEL analysis of the sham-operated mice, Sch B treated mice and the untreated mice at 3 weeks after MI. The green, TUNEL-positive cells are seen merged with blue DAPI-positive cells at 400×magnification. (B) The cardiomyocyte apoptosis index (number of TUNEL-positive cells per 1000 cells) was significantly lower in the Sch B treated group than in the untreated group. (C) Western blotting analysis of Bax, Bcl-2 and ASK1. GAPDH was used as an internal control. (D) Densitometry for Bax expression normalized to GAPDH. (E) Densitometry for Bcl-2 expression normalized to GAPDH. (F) Analysis of the ratio between the expression of Bcl-2 and Bax. (G) Densitometry for ASK1 expression normalized to GAPDH. n = 6 per group, *P<0.05 versus untreated group, **P<0.01 versus untreated group, ***P<0.001 versus untreated group, +P<0.05 versus sham-operated group, ++P<0.01 versus sham-operated group.