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. 2013 Nov 8;8(11):e80252. doi: 10.1371/journal.pone.0080252

Figure 5. Determination of kinetic parameters for the GTPase activity of P. aeruginosa EF-G1A.

Figure 5

A: Initial velocities for P. aeruginosa EF-G1A in GTPase activity reactions were determined at various concentrations of GTP. The concentration of EF-G1A was held constant at 1.0 μM. The velocities were measured between 1 and 6 min to minimize the chance of measurement of GTP hydrolysis occurring during mixing but before the beginning of the incubation period. The concentrations of GTP were: ♦, 25 μM; ■, 50 μM; ▲, 100 μM; +, 200 μM; ●, 400 μM, ×, 600 μM. B: The data from the initial velocity experiments were used to develop a Lineweaver-Burk plot to determine kinetic parameters for the GTPase activity of P. aeruginosa EF-G1A.