Figure 8. The effect of fusidic acid on the activity of P. aeruginosa EF-G in protein synthesis.
Representative assays of the effect of increasing concentrations of fusidic acid on the ability of EF-G1A and EF-G1B to function in protein synthesis. A) EF-G1A and B) EF-G1B. The concentration of EF-G1A and EF-G1B were 1.0 and 0.2 μM, respectively, and the concentration of ribosomes was 0.2 μM. FA was added to the assay in concentrations from 4 μM to 500 μM. EF-G1A and EF-G1B are represented by filled diamonds (♦) and squares (■), respectively.