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. 2013 Nov 8;8(11):e80252. doi: 10.1371/journal.pone.0080252

Figure 9. RRF and EF-G1A function to reduce the synthesis of poly(Phe).

Figure 9

Representative assays depicting the effect of ribosome release factor (RRF) on the activities of EF-G1A, EF-G1B and a combination of EF-G1A and EF-G1B. A) Assays to determine the effect of RRF on the activity of EF-G1B were as described under “Methods and Materials”, with RRF titrated into the assay between 0.2 and 6.4 μM. The concentration of EF-G1B was 0.2 μM. B) Assays to determine the effect of RRF/EF-G1A on the activity of EF-G1B. The concentration of EF-G1B was the same as in “A” and the concentration of EF-G1A and RRF were 1 and 2 μM, respectively.