Fig. 1.
EXOs activate MZ B cells in the absence of T-cell help. (A) CFSE-labeled splenocytes (8×105/200 μl/well) of NOD females (8–10 weeks old) were stimulated with 20 μg/ml of EXOs in a flat-bottom 96-well plate. Cultured cells were harvested at different times and stained with CD4 or B220 antibodies. (B) B cells were purified from the CFSE-labeled NOD splenocytes with MACS beads coated with anti-B220, and then stimulated with EXOs. (C) CFSE-labeled splenocytes (left) or purified B cells (right) were stimulated with EXOs for 72 h, and stained with antibodies shown. Data shown are from one experiment representative of three performed.