Figure 6.

The strand-passage activity of topo IV and its ability to bind MukB are separable and contribute additively to cell growth. Complementation assays of the C600parc1215 strain are shown grown at 42°C on rich (A) and minimal (B) media. Spotted cultures correspond to 20-fold dilution series taken 1 h after liquid cultures were shifted to the restrictive temperature. For plating, cells were normalized to the culture with the lowest OD_600nm at the time of the temperature shift (OD_600nm≈0.3). Cartoons of ParC constructs assayed, their specific activity relative to WT, and their ability to bind MukB, are illustrated next to each row. Dilutions of cells grown at the permissive temperature (30°C) are shown to the right of cells grown at the restrictive temperature. Time points taken from cultures at time points 30–270 min after the temperature shift are shown in Supplementary Figure S6.