Fig. 5.

Effect of anti-CD4/CD8 mAbs on lung and liver metastases (a) and frequency of tumor cells cloned from DLN (b) in CD200R1KO BALB/c mice receiving 5 × 10⁵ EMT6 tumor cells subcutaneously into the mammary fat pads, followed by surgical resection 15 days later, and immunization with EMT6 with CpG as adjuvant. After immunization mice were divided into groups of nine animals, each receiving either control Ig or anti-CD4/anti-CD8 mAbs (75 μg/mouse iv at 72 h intervals for four doses). Three of each group were sacrificed at 7, 14, and 21 days post-surgery to measure macroscopic tumor metastases in the lung/liver (a). DLN cells were harvested from individual mice and cultured under limiting dilution for 3 weeks to assess the frequency of tumor cells cloned (b). All data represent arithmetic means (±SD) for each group. nd in a indicates no metastases detected; *^,**^,***p < 0.05 relative other groups at day 7/14/21, respectively