Fig. 1.

a–d Unsupervised hierarchical clustering analysis of candidate loci methylation in UNC datasets identifies six methylation patterns. The clustergram is highlighted on the left to display the major clada for each dataset. The colored bar on the right of the clustergram displays the methylation pattern group for either each CpG unit or average methylation per gene (MP1 = yellow, MP2 = dark blue, MP3 = light blue, MP4 = orange, MP5 = purple, and MP6 = green). Hierarchical clustering analysis (HCA) by CpG unit of a 81 tumors and b 53 tumors reveal enrichment of methylation patterns for each cluster. HCA of averaged methylation per locus for c 81 tumors and 33 genes and d 53 tumors and 37 genes show similar clustering groups and methylation patterns compared to clustergrams based on individual CpG units. See online resource 3 for a detailed listing of rows (genes, CpG IDs) and columns (tumor subytpe)