Figure 6.

Cutting the connections between Layer II and deeper layers of the PCtx abolished activation of the DEn and converted the inhibition in Layer III to excitation. Representative images before (A,B) and after (C,D) cutting the slice. Black asterisks indicate spots where representative traces were taken. Yellow Line shows the pixels that were used to follow and quantify the response before and after introducing the cuts. Identical regions were quantified. In (E) we show that the reduced excitation of layer II along four pixels while traces in layer III are inverted and became excitatory. Responses in the DEn were completely abolished. In (F) we show the quantitation of the reduction of excitation of layer II and the conversion of layer III responses to excitatory ones.