Figure 1.

Characterization of cell line over-expressing LH-R in vitro and in vivo: cell proliferation and invasion. (A) Cell proliferation of Hec1A and Hec1A-LH-R cells. Cells (2.5 × 10⁴ cells/100 μl) were cultured in 96-well plates. After 24 h incubation, cell proliferation was evaluated with the colorimetric WST-1 assay (the absorbance value is reported on y axes). Histograms represent the mean of the absorbance value ± SEM (error bars). Statistical analysis was performed with Student’s t test. (B,B′) Cell invasion of Hec1A and Hec1A-LH-R cells. Cell invasion was evaluated using a Boyden chamber system where a Matrigel-coated porous membrane (8 μm pore diameter) was inserted as described in Section “Materials and Methods.” At time 0, recombinant LH was added or not to the medium. The number of migrated cells was determined after 24 h of incubation. Cells were fixed and stained with Diff–Quick staining solution, and counted on the whole migration field at ×40 magnification. In (B) pictures relative to representative membrane for Hec1A and Hec1A-LH-R cells, in the presence or in the absence of LH, are reported. Histograms in (B′) represent the mean of migrated cells ± SEM (error bars). Statistical analysis was performed with Student’s t test.