Table 1.
Genes directly regulated by Asxl1 as determined by differential expression in HSPCs with Asxl1 deletion and directly bound by Asxl1
| Gene | MPs | LSK cells | Anti-Asxl1 ChIP-Seq | ||||
| log2 (fold change) | P-value | log2 (fold change) | P-value | Peak p-value | Fold enrichment | Peak FDR | |
| Tmem87a | 3.91 | 9.60 × 10−6 | 3.11 | 3.68 × 10−6 | 2.78 × 10−7 | 4.21 | 0.29 |
| Pusl1 | 1.54 | 2.21 × 10−7 | 1.30 | 1.29 × 10−3 | 3.04 × 10−8 | 5.24 | 0.15 |
| Ddx23 | 1.45 | 1.15 × 10−10 | 1.09 | 1.79 × 10−3 | 7.45 × 10−15 | 6.93 | 0.09 |
| Pan3 | 1.01 | 1.36 × 10−5 | 0.79 | 8.21 × 10−4 | 4.49 × 10−29 | 9.3 | 0 |
| Srrm2 | 0.86 | 6.02 × 10−8 | 0.98 | 1.61 × 10−6 | 1.85 × 10−12 | 4.49 | 0.06 |
| Dusp6 | 0.80 | 4.11 × 10−4 | 0.63 | 2.45 × 10−3 | 4.48 × 10−30 | 8.43 | 0 |
| Cdca2 | 0.77 | 6.77 × 10−8 | 0.62 | 3.84 × 10−4 | 3.78 × 10−8 | 3.6 | 0.16 |
| Apobec3 | 0.54 | 2.25 × 10−10 | 0.36 | 8.29 × 10−4 | 4.48 × 10−10 | 6.18 | 0.09 |
| Cdc40 | 0.41 | 1.68 × 10−4 | 0.59 | 1.93 × 10−5 | 4.79 × 10−10 | 5.91 | 0.09 |
| Rpl30 | 0.39 | 1.73 × 10−10 | 0.31 | 5.74 × 10−5 | 1.57 × 10−22 | 6.99 | 0 |
| Rad51 | 0.39 | 3.17 × 10−3 | 0.51 | 3.48 × 10−3 | 2.28 × 10−10 | 7.16 | 0.1 |
| Nbr1 | 0.34 | 4.65 × 10−8 | 0.43 | 0.00 | 4.04 × 10−19 | 5.69 | 0 |
| Slc24a6 | 0.30 | 4.66 × 10−4 | 0.82 | 3.24 × 10−10 | 7.52 × 10−11 | 8.09 | 0.1 |
| Dhrs3 | 0.28 | 4.27 × 10−3 | 0.37 | 2.16 × 10−4 | 4.33 × 10−49 | 5.15 | 0 |
| Cdk2 | 0.23 | 1.51 × 10−4 | 0.21 | 3.94 × 10−4 | 1.06 × 10−16 | 7.89 | 0.11 |
| Ifngr1 | 0.18 | 4.59 × 10−3 | 0.38 | 1.99 × 10−4 | 3.02 × 10−20 | 11.89 | 0 |
| Hsp90ab1 | 0.16 | 1.08 × 10−3 | 0.24 | 9.11 × 10−5 | 1.41 × 10−45 | 12.71 | 0 |
| Mtmr9 | −0.44 | 4.53 × 10−4 | −0.47 | 3.62 × 10−4 | 4.07 × 10−11 | 7.19 | 0.11 |
| Ppp1cb | −0.48 | 1.35 × 10−8 | −0.64 | 2.14 × 10−7 | 5.79 × 10−12 | 4.77 | 0.09 |
| Mthfr | −0.51 | 1.65 × 10−3 | −0.47 | 1.21 × 10−3 | 2.47 × 10−9 | 4.82 | 0.11 |
| Ncf4 | −0.52 | 7.21 × 10−9 | −0.66 | 4.38 × 10−5 | 2.16 × 10−18 | 7.53 | 0.07 |
| Slc25a19 | −0.96 | 7.68 × 10−11 | −0.75 | 3.50 × 10−6 | 2.24 × 10−7 | 6.64 | 0.28 |
| Rpl29 | −1.18 | 0.00 | −0.76 | 0.00 | 2.47 × 10−9 | 5.96 | 0.11 |
| Mettl23 | −1.33 | 8.43 × 10−10 | −1.10 | 1.21 × 10−5 | 1.10 × 10−7 | 3.68 | 0.2 |
| Caskin2 | −1.54 | 3.42 × 10−5 | −2.45 | 1.53 × 10−4 | 4.97 × 10−13 | 5.6 | 0.07 |
| Thbs1 | −1.75 | 4.71 × 10−7 | −1.84 | 1.63 × 10−6 | 9.53 × 10−8 | 5.06 | 0.19 |
FDR, false discovery rate. Differentially expressed genes in RNA sequencing of MPs (lineage− Sca-1− c-KIT+ cells) from 1-yr-old Mx1-cre Asxl1fl/fl mice relative to Cre− Asxl1fl/fl littermate controls. Differentially expressed genes in RNA sequencing of LSK cells (lineage− Sca-1+ c-KIT+ cells) from 1-yr-old Mx1-cre Asxl1fl/fl mice relative to Cre− Asxl1fl/fl littermate controls. Data derived from anti-Asxl1 ChIP-Seq in WT C57BL/6H BMDMs.