Skip to main content
. Author manuscript; available in PMC: 2014 Feb 4.
Published in final edited form as: Mol Pharm. 2013 Jan 7;10(2):10.1021/mp300477w. doi: 10.1021/mp300477w

Figure 1.

Figure 1

HPLC analyses of phenylalanine (Phe), N-formyl-methionine (fMet), N-formyl-methionyl-leucine (fMet-Leu), and N-formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe) after 5 min incubations of 0.8 mM fMet-Leu-Phe (37 °C) in intestinal segments of duodenum (panel A), jejunum (panel B), ileum (panel C), and colon (panel D) of wild-type mice. All compounds were identified by UV detection using known standards; the retention times were 5.0 min for Phe, 6.5 min for fMet, 14.5 min for fMet-Leu, and 24.0 min for fMet-Leu-Phe. The portal vein was also sampled during a 90 min jejunal perfusion of 0.1 μM [3H]fMet-Leu-Phe (label on Phe*) (panel E). Using different chromatographic conditions (HPLC/radiochemical detection), retention times were 3.5 min for Phe* and 14.5 min for fMet-Leu-Phe* (no signal observed).