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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Nutr Biochem. 2013 Sep 9;24(11):10.1016/j.jnutbio.2013.06.002. doi: 10.1016/j.jnutbio.2013.06.002

Figure 1.

Figure 1

Effect of zinc deficiency on RXR-mediated transcriptional activity in retinoic acid-treated NT2 cells. Cells were grown in zinc adequate (ZA) or zinc deficient (ZD) media for 4 d before transient transfection of an inducible RXR-responsive firefly luciferase reporter and a constitutively expressing renilla luciferase reporter to control for transfection efficiency and then treated with or without retinoic acid (RA) for 24 h. Bars represent mean±SEM (n=3) and represent firefly reporter activity normalized to renilla reporter activity. *ZD+RA is significantly different than ZA+RA at p<0.05. **ZA and ZD are significantly different than ZA+RA at p<0.001 (one-way ANOVA followed by Newman–Keuls Multiple-comparison post hoc test).

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