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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Cancer Res Clin Oncol. 2013 Sep 3;139(10):10.1007/s00432-013-1501-6. doi: 10.1007/s00432-013-1501-6

Figure 1. Proteomics work flow used for autoantibody discovery.

Figure 1

1A: iTRAQ 4plex quantitative proteomics method using pooled serum samples (Table 1A). 1B: Top down proteomics in combination with protein microarray screening method. Protein microarray was generated from 2D separation of ovarian tumor lysate samples and individual serum samples were screened on the protein microarray.