Figure 3.

Western blot analysis of PCNA, BCL-2, cyclin D1, CDK-1, and BAK in EGE cells with and without coculture. EGE and SW872 cells were in coculture for 6 days. Lysates prepared from control and cocultured cells were analyzed by Western blotting with (a) anti-PCNA, (b) anti-BCL-2, (c) anticyclin D1, (d) anti-CDK-1, and (e) anti-BAK antibodies. The intensity of each protein signal was quantified and normalized with corresponding β-actin. Results shown represent three separate experiments with comparable results. *P < 0.05 compared with control.