Figure 2.

Effect of 5′-tailing of aptamer. Apt 2 was synthesized with different polynucleotide (n = 5) tails at the 5′ end (1, untailed Apt 2; 2, 5′-poly-A-tailed; 3, 5′-poly-C-tailed; 4, 5′-poly-G-tailed; 5, 5′-poly-T-tailed) and each was spotted on polyester cloth and then UV cross-linked as before. The strips were saturated with thrombin solution followed by immunoenzymatic assay of bound thrombin as described in Methods. These experiments were repeated in a separate trial with identical results (not shown).