Fig 2A.

Body weight (kg) predicted from age-dependent individual-specific trajectories for CR and CON monkeys (Old-onset). There were significant changes in body weight over time [F(20,311) = 2.52, p = 0.0004]. There was a significant main effect for Sex [F(1,29) = 21.11, p < 0.0001] with males being substantially heavier. Also there were significant Sex-by-Year [F(18,311) = 2.96, p < 0.0001] and Diet-Sex-Year interactions [F(17,311) = 2.37, p = 0.0019] with reductions in body weight due to CR being more prevalent for the male animals. Solid lines represent males; dashed lines represent females. Overall body weight trajectories were based 420 observations for 34 monkeys (84 observations for 8 CON-F; 145 for 10 CON-M; 57 for 7 CR-F; 134 for 9 CR-M). For ages 15–20 years, there were: 12 observations for 5 CON-F; 17 for 7 CON-M; 9 for 5 CR-F; 7 for 4 CR-M. For ages 20–25 years, there were: 38 observations for 8 CON-F; 42 for 10 CON-M; 21 for 5 CR-F; 34 for 8 CR-M. For ages 25–30 years, there were: 21 observations for 5 CON-F; 40 for 8 CON-M; 20 for 4 CR-F; and 41 for 8 CR-M. For ages > 30 years, there were: 46 observations for 8 CON-M; 52 for 6 CR-M and too few to plot for the females. One CR Male that weighed over 14 kg at the start of the experiment had undue influence on the average trend lines for CR Males and therefore the first 5 observations of his data were not used to construct the plot; however, the omission of these observations had virtually no influence on the results of the statistical analyses.