Fig 2C.

Cholesterol predicted from age-dependent individual-specific trajectories for Old-onset CR and CON monkeys. Cholesterol levels increased with age [F(53,774) = 1.54, p = 0.009], and male monkeys had significantly lower levels than females [F(1,24) = 23.60, p < 0.0001]. A significant 3-way Diet-Sex-Age interaction [F(40,774) = 1.53, p = 0.02] indicated that cholesterol levels increased with age for CON males while CR males tended to have a slight reduction in cholesterol. Thus, at older ages (> 30 years), CR male monkeys have significantly lower cholesterol levels as compared to CONs. Overall cholesterol trajectories were based on 994 observations for 28 animals (204 observations for 7 CON-F; 301 for 7 CON-M; 134 observations for 5 CR-F; and 355 observations for 9 CR-Male). For ages 15–20 years, there were: 48 observations for 6 CON-F; 65 observations for 6 CON-M; 21 observations for 4 CR-F; and 32 observations for 4 CR-M. For ages 20–25 years, there were: 91 observations for 5 CON-F; 102 observations for 7 CON-M; 62 observations for 5 CR-F; and 118 observations for 8 CR-M. For ages 25–30 years, there were: 41 observations for 5 CON-F; 70 observations for 6 CON-M; 39 observations for 4 CR-F; and 101 observations for 8 CR-M. For ages > 30 years, there were: 64 observations for 6 CON-M; and 104 observations for 7 CRM; and too few to plot for the females.