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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Leukemia. 2013 May 14;27(10):10.1038/leu.2013.151. doi: 10.1038/leu.2013.151

Table 1.

Sequences of primers used for genotyping, real-time, and 3-primer PCR

Primers used for genotyping
Bcl-x f forward 5’-GTCCTGGCCCTGTCACTTA-3’
Bcl-x f reverse 5’-CCCTTCCCACCTCACTTCCT-3’
BCR/ABL forward 5’-GAGCGTGCAGAGTGGAGGGAGAACA-3’
BCR/ABL reverse 5’-GGTACCAGGAGTGTTTCTCCAGACTG-3’
Cre forward 5’-ACCTGAAGATGTTCGCGATTATCT-3’
Cre reverse 5’-ACCGTCAGTACGTGAGATATCT-3’
tTA forward 5’-GCTAGGTGTAGAGCAGCCTAC-3’
tTA reverse 5’-GGCGGCATACTATCAGTAGTA-3’
Primers used for real-time PCR
Bcl-x forward 5’-ACTTTTGTGGATCTCTACGGGAAC-3’
Bcl-x reverse 5’-CTGAAGAGTGAGCCCAGCAG-3’
Bcl-2 forward 5’-CTTCGCAGAGATGTCCAGTCAG-3’
Bcl-2 reverse 5’-GAACTCAAAGAAGGCCACAATCC-3’
Mcl-1 forward 5’-TTGTAAGGACGAAACGGGACTG-3’
Mcl-1 reverse 5’-TATGCCAGACCAGCCCCTAC-3’
BCR/ABL forward 5’-CGTCCACTCAGCCACTGGAT-3’
BCR/ABL reverse 5’-GGCTTCACTCAGACCCTGAGG-3’
L19 forward 5’-TCTGGTTGGATCCCAATGAGA-3’
L19 reverse 5’-GTCACAGGCTTGCGGATGAT-3’
3-primer PCR (recombination)
Primer A 5’-CGGTTGCCTAGCAACGGGGC-3’
Primer B 5’-CTCCCACAGTGGAGGACCTCG-3’
Primer C 5’-TCAGAAGCCGCAATATCCCC-3’