Figure 1.

Members of the RLR family of viral sensors are expressed in the CNS following in vivo infection with VSV. Mice were infected intranasally with VSV (1 × 10⁷ PFU) and at 5 days post infection, protein isolates were prepared from whole brain tissue homogenates (Brain) or isolated mixed glial cells (Glia) and RIG-I and MDA5 protein expression were determined by immunoblot analyses. Immunoblots shown are representative of three separate experiments. For comparison purposes RLR protein expression in spleen tissue protein isolates is shown (SPL).