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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: J Neurovirol. 2008 Nov;14(6):10.1080/13550280802337217. doi: 10.1080/13550280802337217

Figure 5.

Figure 5

Primary murine microglia constitutively express downstream regulators of RLR function and such expression is modulated following viral challenge. Cells (2 × 106) were untreated (0) or exposed to VSV (ratios of viral particles to cells: 0.001:1, 0.01:1, 0.1:1, 1:1, and 10:1). (A) At 4 h following infection, mRNA was isolated and real-time PCR performed to determine the level of expression of mRNA encoding LGP2. Data are presented as the mean ± SEM of six experiments normalized to the expression of G3PDH. Asterisk indicates a statistically significant difference from uninfected microglia. (B) At 12 and 24 h post infection, whole-cell protein isolates were prepared and LGP2 protein expression was determined by immunoblot analyses. Immunoblots shown are representative of four separate experiments. For comparison purposes, RLR protein expression in spleen tissue protein isolates is shown (SPL). (C) At 4 h following infection, mRNA was isolated and real-time PCR performed to determine the level of expression of mRNA encoding IPS-1. Data are presented as the mean ± SEM of three experiments normalized to the expression of G3PDH. Asterisk indicates a statistically significant difference from uninfected microglia.