Astrocytes express a downstream regulator of RLR function. Cells (2 × 106) were untreated (0) or exposed to VSV (ratios of viral particles to cells: 0.001:1, 0.01:1, 0.1:1, 1:1, and 10:1). At 8 and 24 h following infection, mRNA and whole-cell protein isolates were prepared, respectively. Real-time PCR was performed to determine the level of expression of mRNA encoding LGP2 and LGP2 protein expression was determined by immunoblot analyses. Real-time PCR data are presented as the mean ± SEM of five experiments normalized to the expression of G3PDH. An asterisk indicates a statistically significant difference from uninfected astrocytes. Immunoblots shown are representative of four separate experiments.