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. 2013 Oct 31;2013:347618. doi: 10.1155/2013/347618

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Copyright © 2013 Young Hyun Park et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effect of the gelatin concentration of gelatin-coated two-dimensional (2D) matrix culture dishes on the number and colony size of colony forming unit-fibroblasts (CFU-Fs) derived from mesenchymal stem cells obtained from rat bone marrow primary cell populations. Bone-marrow-derived primary cells were seeded onto culture plates coated with 0, 0.1, 0.5, 1 and 2% (wt/v) gelatin and cultured for 7 days. The CFU-Fs were analyzed using the Image and Microscope Technology (IMT) solution software under an inverted microscope. (a) The CFU-Fs had a typical fibroblastic morphology and were stained by crystal violet. (b) A significant increase in the number of CFU-Fs was detected in bone-marrow-derived mesenchymal stem cells (BM-MSCs) cultured on 0.5, 1 and 2% (wt/v) gelatin matrices. (c) The CFU-Fs with the largest diameters were those of BM-MSCs cultured on 1% (wt/v) gelatin matrix. All data shown are means ± S.D. of four independent experiments. Scale bar (large), 400 μm. Scale bar (small), 1 cm. ^∗,∗∗ P < 0.05.